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Greiner Bio
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Image Search Results
Journal:
Article Title: Overexpression of the ?-2,6-Sialyltransferase in MDCK Cells Increases Influenza Virus Sensitivity to Neuraminidase Inhibitors
doi: 10.1128/JVI.77.15.8418-8425.2003
Figure Lengend Snippet: Binding of the linkage-specific lectins SNA and MAA to cell surface receptors of SIAT1-transfected and parent MDCK cells. Top panel, MDCK-SIAT1 cells (shaded profiles) and MDCK cells (open profiles) were incubated with DIG-labeled lectins followed by incubation with fluorescein isothiocyanate-labeled anti-DIG antibodies and subjected to FACS analysis. Bottom panel, preparations of cell plasma membranes adsorbed in the wells of a 96-well plate were incubated with DIG-labeled lectins followed by incubation with peroxidase-labeled anti-DIG-antibodies. The data represent ratios of absorbencies in the wells coated with membranes of MDCK-SIAT1 cells to those in wells coated with membranes of MDCK cells.
Article Snippet: In brief, viruses adsorbed in the wells of
Techniques: Binding Assay, Transfection, Incubation, Labeling
Journal:
Article Title: Overexpression of the ?-2,6-Sialyltransferase in MDCK Cells Increases Influenza Virus Sensitivity to Neuraminidase Inhibitors
doi: 10.1128/JVI.77.15.8418-8425.2003
Figure Lengend Snippet: Effect of oseltamivir carboxylate on the formation of virus foci in parallel cultures of MDCK (M) and MDCK-SIAT1 cells (ST) infected with the same virus multiplicity. Experiments were performed in 96-well plates as described in Materials and Methods using clinical virus isolates A/Sydney/5/97 (H3N2) (top panel), A/Memphis/14/96-M (H1N1) (middle panel), and B/Memphis/25/99 (bottom panel). The infection time was 24 h for the type A viruses and 48 h for the type B virus.
Article Snippet: In brief, viruses adsorbed in the wells of
Techniques: Infection